Researcher 2

Applicants are invited to apply for full-time Researcher 2 positions in the laboratory of Michael Lipscomb for highly motivated candidates interested in basic and translational studies in immunology. For more information, please visit: https://med.umn.edu/bio/department-of-pharmacology/michael-lipscomb Current NIH funded projects in the Lipscomb laboratory focus on delineating the immunomodulatory mechanisms that govern antigen presenting myeloid cell functions and the development of innovative immunotherapies to combat intracellular pathogens and alleviate autoimmune and immunodeficiency disorders. Candidates will engage in laboratory approaches employing use of multipanel flow cytometric analyses and sorting, qPCR arrays and scRNAseq transcriptome profiling, protein-protein interaction and signaling assays, multiplex luminex analyses, biomaterial organoid assembly and delivery, and RNAi and Crispr-Cas9 gene editing, among other techniques, to answer questions related to immunobiology. Studies use knockout/in transgenic mouse models for in vivo and ex vivo analyses of immune cell functioning and development under both steady state and inflammatory settings.

The Lipscomb lab is dedicated to diversity, equity and inclusion. The promote and recognize principles of fairness and equity in relation to, and across, intersections of race, age, color, disability, faith, religion, ancestry, national origin, citizenship, sex, sexual orientation, social class, economic class, ethnicity, gender identity, gender expression, and all other identities represented among our diverse communities.

Primary responsibilities:

Specific technical/bench-work research (60%):
- Assist in design and performing in vivo and ex vivo immune-based studies/experimentations using mice
model systems under guidance and direction of principal investigator
- Analyze data collected from experiments. Assist research team members with preparing data in an
easily readable format and prepare graphs, charts, tables and reports of data and the scientific findings
- Assist in writing routine summary reports on results of work and confers with supervisors on
interpretation of results and avenues of investigation
- Manage animal colony and genotyping, which includes screening, weaning, tagging and genotyping
- Perform basic molecular analyses including PCR, qPCR, DNA extraction, design and execution of
molecular cloning, CRISPR/Cas9, primer design.
- Tissue culture work, processing of tissues and blood samples, virus and bacterial quantification and
propagation and RNAi mediated gene silencing approaches for experiments
- Usage of major research instrumentation for readout indices to determine functional outcomes
- Supports scientists in the laboratory and conduct investigations or experiments in an assigned area to
aid in the solution of a research problem (standard immunology techniques, western blot, PCR/qPCR,
tissue culture work, learning and applying new techniques as needed etc)
- Independent research at the discretion of the principal investigator.

General laboratory management duties (30%):
- Perform routine genotyping for maintenance and management of animal colonies
- Make standard laboratory reagents (PBS and other buffers, LB media, Agar Plates, DMEM etc), keep
the laboratory stocked and clean, and maintain all laboratory equipment.
- Perform routine maintenance on major research equipment and organize and setup equipment for use
- Manage day to day laboratory operations (shipping and receiving, ordering supplies, inventory,
supervising undergraduate student workers).
- Perform everyday tasks such as laboratory cleanup (using aseptic techniques), replenishing supplies
as needed, remove hazardous and chemical waste
- Maintain a clean and orderly work environment
- Follow established biological, chemical and physical safety procedures and train new personnel

Other duties as assigned (10%):
- Preparing data for presentation, publication, and grant proposals
- Guiding, training, assisting and advising junior researchers and students
- Requires the ability to work occasional weekend hours

All required qualifications must be documented on application materials

Required Qualifications:
- B.S. in any biological, biomedical or biochemistry-related field(s) and 1+ years' research-related experience in immunology studies [OR] a combination of education and experience to equal 5+ years
- Willingness and ability to work with mice in research
- Ability to multitask, organize work flow, and prioritize goals.
- Knowledge of principles, concepts, practices and methods in biomedical research to solve relatively
complex research-related problems and work assignments

Preferred Qualifications:
- Prior experience in small animal (i.e. mice) handling, genotyping and tissue culture work
- Knowledge and experience in use of flow cytometry, Luminex/ELISA, microscopy and qPCR
- Experience in mammalian cell culture, virus/bacterial propagation, and molecular cloning
- Strong interpersonal and written and verbal communication skills
- Past experience working in collaborative environments

The Department of Pharmacology at the University of Minnesota is one of six basic science departments in the Medical School. The well-funded research programs of our primary faculty members focus on understanding and developing better treatments for cancer, addiction and the opioid crisis, cardiovascular disease, infectious disease, and metabolic syndrome.

Working at the University

At the University of Minnesota, you'll find a flexible work environment and supportive colleagues who are interested in lifelong learning. We prioritize work-life balance, allowing you to invest in the future of your career and in your life outside of work.

The University also offers a comprehensive benefits package that includes:

• Competitive wages, paid holidays, and generous time off
• Continuous learning opportunities through professional training and degree-seeking programs supported by the Regents Scholarship
• Low-cost medical, dental, and pharmacy plans
• Healthcare and dependent care flexible spending accounts
• University HSA contributions
• Disability and employer-paid life insurance
• Employee wellbeing program
• Excellent retirement plans with employer contribution
• Public Service Loan Forgiveness (PSLF) opportunity
• Financial counseling services
• Employee Assistance Program with eight sessions of counseling at no cost

Please visit the Office of Human Resources website for more information regarding benefits.

Applications must be submitted online. To be considered for this position, please click the Apply button and follow the instructions. You will be given the opportunity to complete an online application for the position and attach a cover letter and resume.

Additional documents may be attached after application by accessing your "My Job Applications" page and uploading documents in the "My Cover Letters and Attachments" section.

To request an accommodation during the application process, please e-mail employ@umn.edu or call (612) 624-UOHR (8647).

The University recognizes and values the importance of diversity and inclusion in enriching the employment experience of its employees and in supporting the academic mission. The University is committed to attracting and retaining employees with varying identities and backgrounds.

The University of Minnesota provides equal access to and opportunity in its programs, facilities, and employment without regard to race, color, creed, religion, national origin, gender, age, marital status, disability, public assistance status, veteran status, sexual orientation, gender identity, or gender expression. To learn more about diversity at the U: http://diversity.umn.edu.

Any offer of employment is contingent upon the successful completion of a background check. Our presumption is that prospective employees are eligible to work here. Criminal convictions do not automatically disqualify finalists from employment.

The University of Minnesota, Twin Cities (UMTC)

The University of Minnesota, Twin Cities (UMTC), is among the largest public research universities in the country, offering undergraduate, graduate, and professional students a multitude of opportunities for study and research. Located at the heart of one of the nation's most vibrant, diverse metropolitan communities, students on the campuses in Minneapolis and St. Paul benefit from extensive partnerships with world-renowned health centers, international corporations, government agencies, and arts, nonprofit, and public service organizations.