Research Technologist 1 - Transgenic and Targeted Mutagenesis Lab

Department: MED-Center for Genetic Med
Salary/Grade: NEX/9

Target hiring range for this position will be between $18.42-$23.02 per hour. Offered salary will be determined by the applicant's education, experience, knowledge, skills and abilities, as well as internal equity and alignment with market data.

Marketing Statement:

Are you looking to kick-start or continue your career as a research technologist in a cutting-edge laboratory performing genome editing in mice? Are you curious about CRISPR, how is it applied to generate mouse models and learn from the experts how to do it yourself? Our Core facility, Transgenic & Targeted Mutagenesis Laboratory (TTML) at Feinberg School of Medicine, is looking for a highly motivated individual who will support the core's operations in generating, characterizing and preserving mutant mouse strains. The mouse lines we generate are used by research labs across Northwestern, and beyond, to study development, understand the mechanisms underlying disease, and contribute to efforts aiming at finding cures to a battery of debilitating diseases. This job is ideal for a detailed-oriented and organized individual who loves to learn, be challenged, enjoys working as part of a team but also independently, has manual dexterity, and good communication skills. Our team is interactive, supportive and values strong relationships that foster personal and career development. Additionally, the lab is located in newly opened facilities at Northwestern Feinberg School of Medicine which is ideally located in downtown Chicago steps away from Lake Michigan. Although we welcome applicants with any level of experience (eg working with mice, microinjection or survival surgery), we are willing to, and experienced in, training individuals at the start of their career. If you think this is a great match for your career goals, please apply today!

Job Summary:

Conducts tests and procedures; sets up and operates equipment; records observations and measurements and collects and compiles data in a research lab; requires a practical understanding of a limited number of standard scientific techniques

Specific Responsibilities:

• Follows pre-defined protocols, policies, procedure, regulations, Good Clinical and Good Laboratory Practices and conducts standard and repetitive experiments and completes basic trouble-shooting techniques.
• Sets-up, operates and maintains standard lab equipment.
• Using an entry level of skill, completes the following activities

Molecular Biology:

• DNA, RNA, Protein isolation, purification and expression Southern, Northern, Western blotting
• PCR, RT-PCR
• Plasmid preparation
• Cloning, mapping and sequencing of DNA
• Recombinant virus production and purification

Cellular Biology:

• Cell culture, cell isolation
• Functional studies
• Transfection

Histology:

• Tissue fixation/processing: embedding, preparation of blocks and sectioning, staining
• Immunohistochemistry
• Microscopy

Immunology:

• Flow cytometry
• ELISA, Elispot assay
• Cytokine assay
• Antibody production and purification

Animal:

• Husbandry
• Breeding and screening transgenic mice
• Dissections
• Blood collection
• Surgical procedures
• Database management of colony
• Records test results and may maintain databases.
• Completes standard and routine clerical duties such as copying, filing, data entry, word processing, etc.
• Maintains inventory of reagents, mice and lab supplies

Miscellaneous

Performs other duties as assigned.

Minimum Qualifications:

• HS diploma or equivalent and some basic science courses is required

Benefits:
At Northwestern, we are proud to provide meaningful, competitive, high-quality health care plans, retirement benefits, tuition discounts and more! Visit us at https://www.northwestern.edu/hr/benefits/index.html to learn more.

Work-Life and Wellness:
Northwestern offers comprehensive programs and services to help you and your family navigate life's challenges and opportunities, and adopt and maintain healthy lifestyles.
We support flexible work arrangements where possible and programs to help you locate and pay for quality, affordable childcare and senior/adult care. Visit us at https://www.northwestern.edu/hr/benefits/work-life/index.html to learn more.

Professional Growth & Development:
Northwestern supports employee career development in all circumstances whether your workspace is on campus or at home. If you're interested in developing your professional potential or continuing your formal education, we offer a variety of tools and resources. Visit us at https://www.northwestern.edu/hr/learning/index.html to learn more.

Northwestern University is an Equal Opportunity Employer and does not discriminate on the basis of protected characteristics, including disability and veteran status. View Northwestern's non-discrimination statement. Job applicants who wish to request an accommodation in the application or hiring process should contact the Office of Civil Rights and Title IX Compliance. View additional information on the accommodations process.

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